Sustainable protease-assisted extraction of microalgae-derived bioactive compounds

Objectives: To present a novel, sustainable protease-assisted extraction method for microalgae-derived bioactive compounds and evaluate their antioxidant and skin-protective effects. Participants will understand the advantages of enzyme-based extraction over conventional toxic solvent methods, the role of microalgae peptides and lipids in reducing oxidative stress, and their potential applications in anti-aging skincare and aesthetic dermatology. Introduction: This research explores the transformative potential of microalgae as a sustainable powerhouse for anti-aging skincare. By replacing toxic industrial solvents with a patented, protease-assisted extraction method, we have developed a protocol that is 200 times safer for human health and significantly more eco-friendly. Through rigorous biochemical analysis, including SDS-PAGE and GC-MS, we demonstrate how microalgae-derived peptides and lipids effectively scavenge free radicals, reduce oxidative stress, and stimulate collagen synthesis to achieve youthful, radiant skin. Materials / method: Chlorella biomass was subjected to a protease-assisted extraction using marine microbial enzymes. Protein hydrolysates were analyzed by SDS-PAGE to assess molecular weight distribution. Antioxidant activity was evaluated by measuring intracellular reactive oxygen species (ROS) levels in treated HUVEC cells (DPPH, OH*, ORAC). Lipid extracts obtained through sequential ethanol and acetone extraction were analyzed by GC-MS and compared with conventional chloroform/methanol extraction methods to assess yield and composition. Results: Protease-assisted hydrolysis effectively degraded chlorella proteins into low-molecular-weight peptides, as confirmed by SDS-PAGE. The hydrolysates exhibited strong antioxidant activity, with significant scavenging of hydroxyl, DPPH, and peroxyl radicals. In cell models, intracellular ROS levels were markedly reduced in a concentration-dependent manner (p < 0.05). Additionally, the modified extraction method significantly increased lipid yield and improved lipid composition compared to conventional methods, demonstrating enhanced potential for skin-protective applications. Conclusion: Protease-assisted extraction of microalgae provides a safe, sustainable, and efficient approach to obtain bioactive compounds with potent antioxidant and skin-protective effects. These compounds effectively reduce oxidative stress and show promising potential for anti-aging and skin regeneration. This method offers a non-toxic alternative to conventional extraction techniques and supports the development of innovative, eco-friendly solutions in aesthetic dermatology and skincare.