Disclosures

Did you receive any funding to support your research for this TOPIC?
Yes
Please specify entities (individual, company, society): nexgeen

Were you provided with any honoraria, payment or other compensation for your work on this study?
Yes
Please specify entities (individual, company, society): nexgeen

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
Yes
Please specify entities (individual, company, society): nexgeen

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work is presented thanks to the support of: nexgeen

Objectives: İNTRODUCTİON:
The current study introduce a novel approach on the usage of Stromal Vascular Fraction (SVF) in conjunction with fat extract (FE) termed Fat Juice, presenting a comprehensive biochemical analysis and potential relevance of this new combination .
METHODS AND RESULTS : The technique employs a non-enzymatic mechanical method. A biomolecular analyses of the mixture based on the quantity and viability of stem cell, presence and quantification of connective tissue fibers (collagen type I, III, and elastin), interleucine-6, MRC 1 and VEGF is presented. Stem cell isolation and enumerat

Introduction: İNTRODUCTİON:
The current study introduce a novel approach on the usage of Stromal Vascular Fraction (SVF) in conjunction with fat extract (FE) termed Fat Juice, presenting a comprehensive biochemical analysis and potential relevance of this new combination

Materials / method: The technique employs a non-enzymatic mechanical method. A biomolecular analyses of the mixture based on the quantity and viability of stem cell, presence and quantification of connective tissue fibers (collagen type I, III, and elastin), interleucine-6, MRC 1 and VEGF is presented. Stem cell isolation and enumeration were performed on 11 samples. Stem cells average sizes and viability rates were analyzed.

Results: The findings indicate that total stem cell amount range from 0.14 to 1.31 x 10^5, while cell viability rates vary between 20% - 67.9%. IL-6, MRC1 and VEGF protein levels were examined by ELISA method. Elastin, Collagen-I and Collagen-III were analyzed with histological and pathological examinations. Additionally hematoxylin-eosin staining was performed. The staining intensity in cells and fibers where analysed and compared.

Conclusion: Fat juice mixture is an easy to inject concentration of adipocyte/preadipocytes, red blood cells, adipose-derived stem cells, endothelial-derived cells and cell residues. The study observed, quantified and compared between samples the presence of connective tissue proteins, İL-6, MRC1 and VEGF. The results showed potential for usages on skin rejuvenation and regeneration due to the combined properties of the SVF and FE. The authors agree that its easy isolation process and abundant availability Fat Juice is an effective skin quality enhancer widely useful.

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability

Objectives: Objective:
The objective of this lecture is to provide participants with a comprehensive understanding of the evolution of adipose-derived cell isolation techniques, transitioning from chemical to mechanical dissociation methods. The focus will be on exploring how these advancements impact innovation, technology, and regenerative medicine applications.

Learning Outcomes:

Historical Context: Participants will gain insight into the historical development of adipose tissue manipulation techniques, tracing the evolution from chemical dissociation methods to modern mechanical approaches.
Chemical

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability