Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
Yes
Please specify entities (individual, company, society): IMCAS BOARD

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
Yes
Please specify entities (individual, company, society): ARTHREX HORIBA FIDIA HORUS MACOPHARMA REMEDEX

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability

Objectives: Exosomes continue to be a hot topic of discussion in dermatology and aesthetics, offering new opportunities for improving outcomes. However, many folks have come to take sides pro and con. In this talk, I will explore what is new in the literature, how I am using them and delve into the controversies surrounding exosomes in a complex and uncertain regulatory environment. At the conclusion of this learning activities, attendees should be familiar with the latest exosome science and be able to list some of the risks and benefits to incorporating them into practice.

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
Yes
Please specify entities (individual, company, society): BENEV, Viol, ExoCoBio, Allergan, Galderma

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability

Objectives: 1. To share the technology and product information on ExoCoCio
2. Overall information on our products, eg price and others
3. Scientific discoveries including preclinical and clinical studies with our exosome technology

Disclosures

Did you receive any funding to support your research for this TOPIC?
Yes
Please specify entities (individual, company, society): ExoCoBio Inc.

Were you provided with any honoraria, payment or other compensation for your work on this study?
Yes
Please specify entities (individual, company, society): ExoCoBio Inc.

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
Yes
Please specify date...: Patented
and status : Dec 2019

This work is presented thanks to the support of: ExoCoBio Inc.

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
Yes
Please specify entities (individual, company, society): Employee

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
Yes
Please specify date...: patent pending
and status : 2022

This work was not supported by any direct or non direct funding. It is under the author's own responsability

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
Yes
Please specify entities (individual, company, society): BREXOGEN Inc.

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work is presented thanks to the support of: BREXOGEN Inc.

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
Yes
Please specify entities (individual, company, society): kol for dermoaroma

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability

Objectives: 1. Defining Exosomes, significance of cell to cell communication and ongoing research

2. What makes Kimera exosomes different

3. How Kimera does exosome categorization

Introduction: Composition of Exosomes and the heterogeneity of contents do differ amongst various products on the market.

Exosome key surface markers and RNA content is assessed via specialized microsope to delineate and differentiate between exoxomes and random particles

Materials / method: Advances in isolating and characterizing exosomes utiizing the Dstorm microsope by Kimera for detecting nano imaging of the cell wall fo accuracy and super high resolution.

Results: Use of high quality sterile pure product as applied topically has shown the efficacy and safety of exosomes when applied after various laser treatments and injuries to speed up healing and skin regeneration.

Conclusion: Emphasis on the transformative potential of exosomes in regenerative medicine and aesthetics as well as the need for further research to utilize the potential of exosomes for diagnostic and therapeutic indications.

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work is presented thanks to the support of: Kimera Labs

Objectives: Obtener las herramientas para evaluar los productos basados en exosomas del mercado.

Introduction: Presentar evidencia seleccionada.

Materials / method: Búsqueda bibliográfica.

Results: - No todos los exosomas son iguales

Conclusion: - El médico es el responsable de conseguir el suficiente conocimiento como para poder elegir sabiamente el producto que utilizará.

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
Yes
Please specify entities (individual, company, society): MCT

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
Yes
Please specify date...: Concedida
and status : MCT

This work was not supported by any direct or non direct funding. It is under the author's own responsability

Objectives: Exosomes are small extracellular vesicles ranging from 30-150 nm in diameter, playing a crucial role in cell communication, tissue repair, and regeneration. In these collective studies an innovative therapy method based on Platelet Rich Plasma (PRP) to induce the release of billions of vesicles by stressing platelets and other blood cells within PRP. Through a specialized protocol and devices, this method enhances platelet activation, providing a plasma rich in exosomes derived from platelets. This approach finds extensive applications in aesthetic, medical and therapeutic treatments.

Introduction: This study aims to develop a PRP variant targeted for aesthetic and medical applications. The treatment method delivers effective outcomes in various areas such as skin regeneration and hair loss. Notably, this method is entirely autologous, utilizing exosomes derived from the patient's own platelets. This approach ensures safety by being closed to horizontal gene transfer, thereby eliminating the risk of immune reactions or other complications associated with foreign biological materials. Additionally, the final milieu is fully injectable for the use of clinical applications.

Materials / method: Using the Microlyzer 600µm (T-LAB – Turkey), blades were employed to stress the cells, inducing the secretion of billions of vesicles and promoting platelet activation. The shear stress created by the device applied mechanical forces to the platelets, leading to their activation and subsequent exosome production. An Advanced PRX Tube was used to further facilitate the secretion of billions of vesicles by cells and the activation of platelets. This tube generated vortices through varying G-forces, continuing the activation process.The protocol steps ensured the release of production of EVs.

Results: Laboratory findings confirm that this autologus exosome method achieves 4.5×10^9 particles/mL of vesicles, with an average vesicle size of 116.6 nm. This particle count is significantly higher than that of most exosome products currently available on the market, indicating the superior efficacy of our method. Analysis of CD markers reveals: CD9: 99.6%, CD45: 0.3%, CD41: 99.4%, CD61: 98.6%, CD81 : 98.5%, Viability: 98.9%. The results regarding less presence of leukocytes and high presence of plt-derived EVs reinforces the purity and targeted effectiveness of our method.

Conclusion: The results obtained with autologous plt-derived exosomes rich plasma offers higher yielded exosomes content within the finally characterized milieu. Due to its nature, apart from widely offered different source of exosome alternatives, autologous platelet-derived exosomes rich plasma technique poses no risk of allergic reactions post-injection, making it a safe and effective treatment option in aesthetic and medical applications.

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
Yes
Please specify entities (individual, company, society): T-LAB, Founder

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability