Resumen

Objectives: İNTRODUCTİON: The current study introduce a novel approach on the usage of Stromal Vascular Fraction (SVF) in conjunction with fat extract (FE) termed Fat Juice, presenting a comprehensive biochemical analysis and potential relevance of this new combination . METHODS AND RESULTS : The technique employs a non-enzymatic mechanical method. A biomolecular analyses of the mixture based on the quantity and viability of stem cell, presence and quantification of connective tissue fibers (collagen type I, III, and elastin), interleucine-6, MRC 1 and VEGF is presented. Stem cell isolation and enumerat Introduction: İNTRODUCTİON: The current study introduce a novel approach on the usage of Stromal Vascular Fraction (SVF) in conjunction with fat extract (FE) termed Fat Juice, presenting a comprehensive biochemical analysis and potential relevance of this new combination Materials / method: The technique employs a non-enzymatic mechanical method. A biomolecular analyses of the mixture based on the quantity and viability of stem cell, presence and quantification of connective tissue fibers (collagen type I, III, and elastin), interleucine-6, MRC 1 and VEGF is presented. Stem cell isolation and enumeration were performed on 11 samples. Stem cells average sizes and viability rates were analyzed. Results: The findings indicate that total stem cell amount range from 0.14 to 1.31 x 10^5, while cell viability rates vary between 20% - 67.9%. IL-6, MRC1 and VEGF protein levels were examined by ELISA method. Elastin, Collagen-I and Collagen-III were analyzed with histological and pathological examinations. Additionally hematoxylin-eosin staining was performed. The staining intensity in cells and fibers where analysed and compared. Conclusion: Fat juice mixture is an easy to inject concentration of adipocyte/preadipocytes, red blood cells, adipose-derived stem cells, endothelial-derived cells and cell residues. The study observed, quantified and compared between samples the presence of connective tissue proteins, İL-6, MRC1 and VEGF. The results showed potential for usages on skin rejuvenation and regeneration due to the combined properties of the SVF and FE. The authors agree that its easy isolation process and abundant availability Fat Juice is an effective skin quality enhancer widely useful.