Resumen

Objectives: To evaluate the efficacy and safety of in vitro expanded ADSCs for facial rejuvenation in Thai participants, focusing on wrinkle reduction, pore size reduction, texture improvement, and skin elasticity. Introduction: Facial aging is characterized by wrinkles, reduced elasticity, and surface irregularities, all of which negatively affect appearance and quality of life. Current treatments provide only partial or temporary improvement. Adipose-derived stem cells (ADSCs), belonging to the mesenchymal stem cell family, possess regenerative abilities through paracrine signaling, stimulation of fibroblasts, and extracellular matrix remodeling. Their application in aesthetic medicine is therefore of increasing interest. Materials / method: Thirty Thai participants aged 18–60 years with no facial treatment in the past six months were recruited. From each subject, 100 ml of abdominal fat was harvested, processed, and expanded to yield ADSCs at a concentration of 1 million cells/ml. A total of 5 ml was injected beneath facial areas in two sessions one month apart. Clinical assessments included Antera 3D imaging for texture, wrinkles and Pore size and Cutometer measurements for elasticity at baseline and up to six months. Results: Antera analysis showed significant improvement in boxcar scars, with texture enhanced by 15.43% at one month and 14.47% at three months. Wrinkles improved 13.67% at one month and 12.13% at three months, while pore size decreased 24.88% at one month. Skin elasticity testing demonstrated increased firmness at two months after the second injection, improved Immediate skin retraction ability at one month, and consistent overall elasticity improved at one, two, three, and six months after the second injection. Conclusion: The findings suggest that ADSCs, as mesenchymal stem cells, may support dermal regeneration and skin repair through cellular and paracrine mechanisms. Clinical improvements were observed in wrinkles, texture, and elasticity, indicating potential benefit in facial rejuvenation. However, given the limited sample size and follow-up duration, these results should be interpreted cautiously. Further controlled trials are needed to confirm efficacy, optimize protocols, and evaluate long-term safety before routine clinical application.