Resumo

Objectives: This single-center, prospective, open-label randomized study aimed to produce a repeatable procedure for isolating platelet-rich plasma (PRP), maximizing platelet recovery and concentration parameters from the collected material, and to assess the effectiveness of autologous platelet-rich plasma (concentrated platelet rich plasma C-PRP and platelet rich plasma low centrifugation concept PRP LCC) and platelet-rich fibrin (injectable platelet rich fibrin I-PRF and fluid platelet rich fibrin F-PRF) injections on skin density and thickness in facial aesthetic treatments. Introduction: The main benefit of platelet-rich plasma preparations is their safety, biocompatibility, and effectiveness in a wide range of medical indications, as well as lack of serious side effects, which distinguishes this therapy from other approaches. Unfortunately, to date, there is no consensus or clear regulations regarding the quality of plasma preparations, which would translate into the quantity of platelets, leukocytes, growth factors, fibrin, or other biologically active factors, and erythrocytes. Materials / method: The study consisted of two stages: a laboratory and a clinical one. In the laboratory phase, 20 volunteers participated. In the clinical phase another 20 volunteers had blood drawn from the peripheral vein in a single collection procedure, successively into two tubes: a 10 mL tube containing 1 mL of anticoagulant (sodium citrate) and an empty 10 mL polycarbonate tube. The material was centrifuged.The separate centrifugation procedures allowed to obtain 4 plasma fractions which were then injected in the facial skin. Results were evaluated by ultrasound measurements( skin density & thickness). Results: The proposed preparation protocol confirms the effectiveness of PRP and PRF in improving skin density and thickness in specific facial areas. Ultrasound measurements and statistical analysis revealed a significant increase in skin density of the cheek and forehead areas, as well as in density and thickness of the lower eyelid skin, indicating the effectiveness of these treatments in enhancing skin quality. The high levels of patient satisfaction, as reflected in the Global Aesthetic Improvement Scale and the willingness of participants to recommend the treatment, affirm the positive outcome. Conclusion: This approach (one blood drawing, 4 plasma fractions) in terms of obtaining optimal amount of PRP/PRF and effectiveness of the therapy - increase of skin density and thickness, offers a promising direction for future treatments in aesthetic medicine, enhancing both the effectiveness and the convenience of such therapies. This work was published recently: Dermatologic Therapy, 2024, 8649287, 12 pages, 2024. https://doi.org/10.1155/2024/8649287.