Resumo

Objectives: Exosomes and related technologies are booming research fields in regenerative medicine. Recent discoveries unveiled their potential in improving cellular health-span and skin aesthetic quality. This lecture will help the audience gain insights on: 1). Updated science-based evidence on Exosomes, Extracelullar vesicles derived from non-mammalian species, Bio-engineered vesicles, and their potential application in regenerative aesthetics and health-span improvement. 2). New imaging techniques to characterize and visualize exosomes. 3). Biomarkers and longevity hallmarks being investigated. Introduction: Exosomes, small vesicles carrying bioactive molecules such as RNAs and signaling peptides, are showing promise in regenerative medicine and aesthetics. Early research suggests they may rejuvenate tissues, accelerate wound healing, and reduce inflammation. In addition, recent discoveries demonstrated their potential to impact human longevity hallmarks. Exosome-related technologies such as extracellular vesicles from various species, bioengineered vesicles, and liposomal encapsulated biomaterials are emerging, bringing new avenues for therapeutic and aesthetic applications. Materials / method: Reviews of updated clinical trials and scientific literatures will be shared for knowledge updates. Human dermal fibroblasts were cultured, conditioned media was harvested and concentrated, Nanotracker was adopted to visualize and quantify exosomes and vesicles from different species. Transmission electron microscope was used to visualize more detailed vesicle structures. The membrane of exosomes was tagged with a fluorescent dye in order to visualize the secretion and uptake process between live cells. qPCR and multiplex were used to investigate biomarkers for regeneration and longevity. Results: Both human mesenchymal stem cell-derived exosomes and extracellular vesicles from non-mammalian source could impact biosignatures of cellular activities, including cell signaling, proliferation, migration, and beyond. Differentiated biomarkers for regeneration and cellular health were impacted by vesicles derived from various species, or through different manufacturing processes. While these findings are promising, there is a lack of standardized process of exosome production, characterization, and double-blind placebo-controlled clinical trials, future research and investigation is required. Conclusion: Exosomes and adjacent technologies have demonstrated promising results in regenerative aesthetics and tissue health-span. Despite these promising avenues, comprehensive scientific and clinical validation is needed to fully understand the efficacy and safety of exosome-related treatments in promoting regeneration, longevity, and health benefits.​