Objectives: The brow area is an important aesthetic unit. Accurate anatomical understanding of the brow is important for clinical treatments related to this region. Due to the complexity of the brow area and limitations of methods, there are still some controversies regarding description of its musculature. In this study, we aimed to reveal the detailed brow musculature with micro-computed tomography (micro-CT) combined with histological staining. Our study deepens the understanding of the precise anatomy of the brow area and helps guide clinical practice.