摘要

Objectives: Botulinum Toxin type A and exosomes have been cited for their capabilities of synthesizing collagen and elastic fiber. However, the delivery of these compounds has been focused on the dermis level. This clinical study explores a unique protocol for delivering a cocktail of exosomes and botulinum toxin non-invasively to the dermal-epidermal junction. Introduction: The dermal-epidermal junction (DEJ) intersects the epidermis and the dermis. The functions of the DEJ are epidermal-dermal adherence, mechanical support for the epidermis, and a gate for the passage of cells and molecules across the junction. With aging, the altered composition of proteins in the skin, such as collagen, integrin, and laminin, affects the physiology of the DEJ. This flattening of the DEJ reduces its surface area leading to the increased fragility of aged skin. Materials / method: This clinical study explores a unique protocol for delivering a cocktail of exosomes and botulinum toxin non-invasively to the dermal-epidermal junction. Results: The results showed the increased synthesis of collagen and elastic fibers at the junction, improving skin elasticity and firmness. This also resulted in enhanced epidermal-dermal adherence, which provided better mechanical support for the skin and reduced the risk of skin tears or injuries. Furthermore, the increased passage of cells and molecules across the junction potentially improved skin hydration and nutrient delivery. The overall skin quality, including skin tone, texture, and radiance, improved significantly, resulting in increased patient satisfaction with the treatment. Conclusion: In conclusion, delivering a combination of exosomes and botulinum toxin to the dermal-epidermal junction is a promising non-invasive technique for improving the physiological function of the DEJ and enhancing overall skin quality. This was evidenced by self-reported improvements in skin appearance and reduced signs of aging. Further studies may help optimize the protocol for different non-invasive procedures and improve our understanding of the interactions with energy-based devices. Ultimately, this technique can potentially become a new paradigm in anti-aging treatment.