摘要

Objectives: - In vivo investigations of the restructuring effect on the skin barrier function and breathing effect of the new skin healing photoprotector - In vivo analysis of the impact of the photoprotector on skin microbiome - Clinical study after laser procedure to evaluate the tolerance and efficacy of the photoprotector in wound healing, especially on post-inflammatory hyperpigmentation Introduction: The use of a photoprotector is commonly practiced following certain aesthetic procedures, in particular to prevent post-inflammatory hyperpigmentation (PIH). The objective of these studies was to demonstrate the tolerance and efficacy of a new skin healing SPF50+ photoprotector, formulated based on the ecobiological approach which considers the skin as a living ecosystem interfacing with its internal and external environment, and preserving its natural biology. Thus, four experimental studies targeting the biological mechanisms of the skin and one clinical study were carried out. Materials / method: The protective and breathing effects of the photoprotector were investigated via two TEWL studies and by measuring the transcutaneous O2 and CO2 partial pressures (radiometer). Then, skin microbiome was analyzed via 16S rRNA gene sequencing after disinfection. Finally, the tolerance and efficacy of the product (twice/day, 14 days) were evaluated in 32 subjects after post-laser in a non-comparative clinical study. An overall scar score (3 subscores: inflammation, scar appearance, functional signs) was performed by a dermatologist in addition to instrumental measurements (TEWL, Visia). Results: A significant improvement of the TEWL was observed after blotting paper (21.4%) and after rubbing (7.8%), without variation in transcutaneous O2 and CO2 pressures. The microbiome analysis showed a diversity restoration from 3hrs after disinfection, unlike the control. Finally, in addition to no adverse event, the photoprotector significantly reduced the overall scar score by -13.7% immediately after laser up to -57.1% at D14 (in particular PIH -21.8%) vs D0. Moreover, a significant reduction of the pigmentation (-17.9%) was observed vs D0 and of the TEWL (-6.5% from D1 vs after laser). Conclusion: In conclusion, this new skin healing SPF50+ photoprotector designed using an ecobiological approach, promotes the natural healing process while protecting against UV rays, in the respect of the skin's ecosystem, especially its microbiome, for optimal healing without hyperpigmentation.