摘要

Objectives: 1: which kinds of wounds can be healed by stem cell therapy? 2: How can we do this treatment? Introduction: A biocompatible and readily available wound dressing for emergencies has been shown to be more cost-effective, while also reducing the risk of immune system-mediated reactions. Materials / method: In this project, we investigated the use of a fresh blood-derived matrix as a wound dressing, based on a 3D drug-loaded Plasma-rich Fibrin (PRF) scaffold, to support the transplantation of autologous stem cells for regenerating skin tissues lost due to burns. PRF scaffold was prepared from venous blood, and adipose tissue-derived stem cells (ADSCs) were isolated from the visceral fat tissue of rats. Following in vitro analysis, PRF gel and ADSCs were transplanted onto second-degree deep burn wounds on the backs of ratswound size measurements were conducted on days 5, 10, 15, and 21 Results: PRF gel,as a cyto-compatible scaffold with the potential for antibacterial drug release(sustained for up to 3 days, with up to 89.7%release), significantly enhanced the healing process in the treatment group.On day 15,a reduced wound size, mature skin cells, and well-organized, thicker collagen fibers were observed in the histopathology of the PRF-treated groups, which scored an average of(2.83±0.04) out of 3 for overall histopathological parameters.The greatest wound contraction was seen in the scaffold-treated groups(5.32±0.61mm2), compared with the control group (7.96 ±  0.82 mm2)(p < 0.05) Conclusion: PRF scaffold and ADSCs have the potential to serve as an effective biological wound dressing for burn wounds, accelerating the healing process and offering an alternative to traditional skin grafting