Objectives: To learn an easy and fast way to harvest lipoaspirate, isolate ADSC using an office based disposable system, and deploy these regenerative cells for different indications and anatomical regions.
Introduction: Adipose tissue derived stromal vascular fraction (SVF) is a reliable source of stromal vascular cells with regenerative potential in plastic surgery and other specialties. Although the gold standard, this enzymatic isolation method is expensive, requires expert personnel, raises legal and administrative concerns on the alteration of cell characteristics. We use a mech isolation system with comparable results
Materials / method: A total of 40 ml lipoaspirate was harvested from 60 women undergoing liposuction and was submitted to conventional enzymatic digestion for SVF isolation or mechanical digestion using a closed unit harnessing 3 ports with blades, followed by buffer incubation and centrifugation. Culture of the SVFs and flow cytometry were performed.
Results: The SVF cell yield obtained by enzymatic digestion was significantly higher 3,38 x 106/ml (±3.63, n=35) than that obtained by mechanical digestion 1,34 x 106/ml (±1.69, n=35), p=0,015. The average cell viability was 82.86% ±10.68 after enzymatic digestion versus 85,86% ±5.74 after mechanical digestion, which was not significant. Mechanical digested SVF expressed 2-fold higher stem cell surface markers compared with enzymatically digested SVF. Mechanical digestion was less time consuming, cost effective and didn't require a specific lab environment.
Conclusion: Mechanically digested SVF was comparable to enzymatically digested SVF in terms of stromal cell composition and viability. With mechanical digestion, we can isolate 30 to 50% SVF cells of that isolated with enzymatic digestion. Clinical outcomes seem very promising. Further studies are underway.
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