Abstract

Objectives: The primary objective of this study was to evaluate the biological activity of a platelet-derived secretome on skin quality improvement using a structured regenerative protocol. Secondary objectives included the assessment of changes in key cutaneous parameters, such as inflammation-related signs, pigmentation homogeneity, pore appearance, hydration, and overall skin texture, through combined clinical, instrumental, and patient-reported evaluations. An additional objective was to investigate the tolerability and safety profile of the platelet-derived secretome by monitoring adverse effects. Introduction: Platelet-derived secretomes represent an emerging paracrine-based approach in regenerative aesthetics, offering biological stimulation without the risks associated with cell-based therapies. Their integration with structured topical regeneration may enhance clinical outcomes while maintaining a favorable safety profile. Materials / method: Ten patients were enrolled in a three-month prospective protocol combining two in-office applications of autologous platelet-derived secretome with a structured topical regenerative regimen. Baseline and follow-up assessments included clinical evaluation, three-dimensional imaging, and biophysical skin measurements. Patient-reported outcomes and adverse effects were monitored using the FACE-Q validated questionnaire at predefined time points. Results: The treatment was well tolerated, with no significant adverse effects reported. Clinical and instrumental evaluations showed a reduction in cutaneous inflammatory signs, associated with an early improvement in skin pigmentation homogeneity and a visible reduction in dilated pores. Patient-reported outcomes confirmed high tolerability and overall treatment acceptance. Conclusion: The combined platelet-derived secretome protocol demonstrated a favorable safety profile and early regenerative effects on skin quality, supporting its role as a promising paracrine-based strategy in regenerative aesthetic medicine.