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FOCO EN S32

Enriching the debate on nano fat

Partner of the session

IAAPS

Indian Association of Aesthetic Plastic Surgeons

India

Sala: Channel 3
Fecha: sábado 17 octubre 2020 de 10:00 a 11:00
Formato: DEBATE > discusión estructurada entre oradores que comparten opiniones sobre un tema debatido.

Moderadores

Prof Katarina A ANDJELKOV

Cirujano Plástico

Serbia

Dr Ashish DAVALBHAKTA

Cirujano Plástico

India

Las presentaciones

Horas	Oradores	Título de la presentación	Resumen	Número
10:00	Dr Ashish DAVALBHAKTA	Welcome message from the IAAPS		107298
10:05	Dr Steven R COHEN	Cell optimized nanofat: what can you do with it?	Ver	107300
10:20	Dr Anmol CHUGH	Debate with Steven Cohen on the preparation of nanofat		107299
10:30	Dr Tunc TIRYAKI	The evolution of stem cell enriched fat injections in plastic surgery: 10 years clinical experience	Ver	107301
10:45	Dr Aniketh VENKATARAM	Debate with Tunc Tiryaki on the treatments by nano fat	Ver	107302
10:55		Round table discussion		107304

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Dr Ashish DAVALBHAKTA

Cirujano Plástico

India

Otro(s) autor(es) :

Welcome message from the IAAPS

Dr Steven R COHEN

Cirujano Plástico

Estados Unidos

Otro(s) autor(es) : Steven R. Cohen MD, Serli Canikyan BS, Tunc Tiryaki MD, Aris Sterodimas MD, Beatriz Nicaretta MD

Cell optimized nanofat: what can you do with it?

Objectives: Explain how different devices make different nanofat products
Discuss the various uses of nanofat as a regenerative product

Introduction: Nanofat, a name coined by Tonnard and Verpaele, describes a stromal vascular fraction (SVF) product that can be injected through a 27 gauge needle. SVF was first used in aesthetic surgery in 2003 by Cohen and Holmes and can be obtained both enzymatically and mechanically. Cell optimized nanofat is a mechanically derived, SVF product that preserves the fibrotic niche of the cells, optimizing the cell counts and hence the regenerative functions of the nanofat. Herein, we describe the how cell optimized nanofat is used in clinical plastic surgery, dermatology and aesthetic gynecology.

Materials / method: Fat is harvested under tumescent anestheia using a cannula with 2 mm holes. The fat is cleaned with normal saline or ringer's lactate and allow to decant. The decanted fat is transferred to a 10 or 20 ml syringe and passed from port 1-2, one time to convert the fat into a 1 mm parcel. Next, this fat is transferred back and forth 10 times from port 2-3 to smooth the product and lastly, the fat is transferred one time from port 3-4, to make the cell optimized nanofat. This nanofat when compared to Tulip's technique of emulsification and filtering, yields almost 2 times the number of SVF cells.

Results: Cell optimized nanofat can be delivered by cannula, by 27 gauge needles, by microneedling devices and as a unique topical biocream, by centrifuging the nanofat and compounding it with a liposomal transport agent and arnica. Cell optimized nanofat is used as component of isolated facial fat grafting to address skin regeneration, in conjunction with facelifting, in addition to fractional laser resurfacing both as a microneedled nanofat as well as a topical biocream. Cell optimized nanofat has been used for difficult wounds anywhere on the body, including areas of radiation.

Conclusion: Cell optimized nanofat is a highly effective biologic compound that can be used in many areas of plastic and reconstructive surgery for facial skin and tissue regeneration, difficult wounds, compromised tissue and a variety of dermatologic conditions especially when associated with autoimmune disorders. Vaginal mucosal regeneration is another promising application and is easily performed during vaginal rejuvenation procedures. Early evidence of signicant improvement in fractional laser outcomes have been demonstrated as has long term fat graft retention.

Declaraciones

¿Ha recibido algún tipo de financiamiento para realizar su investigación sobre esta temática?
No

¿Ha recibido algún pago, honorario u otra compensación por su trabajo acerca de esta investigación?
No

¿Tiene vínculos financieros con alguna entidad que podría llegar a competir estrechamente con los medicamentos, materiales o instrumentos tratados en su investigación?
No

¿Posee o ha solicitado una patente relacionada a los materiales, productos o instrumentos utilizados en su investigación?
No

Este trabajo no cuenta con el apoyo de ningún financiamiento directo o indirecto. El autor asume plena responsabilidad sobre el mismo.

Dr Anmol CHUGH

Cirujano Plástico

India

Otro(s) autor(es) :

Debate with Steven Cohen on the preparation of nanofat

Dr Tunc TIRYAKI

Cirujano Plástico

Turquía

Otro(s) autor(es) :

The evolution of stem cell enriched fat injections in plastic surgery: 10 years clinical experience

Objectives: To learn the usage of ADSC in reconstructive and aesthetic plastic surgery

Introduction: Autologous fat transplantation is increasingly being used for a variety of cosmetic indications. However, the long-term predictability of volume maintenance remains a limitation of fat transplantation, especially in cases of high volume transplantations. To overcome the problems associated with autologous fat transfer we use enzymatic or mechanically isolated autologous ASCs to enhance angiogenesis, improve the survival rate of grafts, and to reduce postoperative atrophy.

Materials / method: Once obtained, the first part the lipo-aspirated fat is digested and ADSC isolated. While the cell separation takes place, the major fat aspiration, transfer and tissue reshaping is done simultaneously. Using mechanic or enzymatic cell separation techniques the ADS's are obtained and injected, using the recently transferred fat as a living scaffold.

Results: This technique was applied in selected 481 patients between 2008 and 2020with a follow-up changing from 8 months to 10 Years. The patients either needed large volume fat transfers or underwent previous traditional lipoinjection procedures without satisfactory results as well as for cosmetic reasons. Cell-enriched grafts ranged in volume from 10 to 390cc per recipient area.

Conclusion: Our results suggest that regenerative cell enriched tissue injections might have significant advantages compared to traditional fat transplantation. These cells are reported to bridge early ischemia secondary to angiogenesis, anti-apoptosis, and modulation of the inflammatory response. This technique may be of particular value to the surgeon while grafting high volumes of fat or when faced with hostile recipient area conditions including fibrosis and post-radiation.

Declaraciones

¿Ha recibido algún tipo de financiamiento para realizar su investigación sobre esta temática?
No

¿Ha recibido algún pago, honorario u otra compensación por su trabajo acerca de esta investigación?
No

¿Tiene vínculos financieros con alguna entidad que podría llegar a competir estrechamente con los medicamentos, materiales o instrumentos tratados en su investigación?
No

¿Posee o ha solicitado una patente relacionada a los materiales, productos o instrumentos utilizados en su investigación?
No

Este trabajo no cuenta con el apoyo de ningún financiamiento directo o indirecto. El autor asume plena responsabilidad sobre el mismo.

Dr Aniketh VENKATARAM

Cirujano Plástico

India

Otro(s) autor(es) :

Debate with Tunc Tiryaki on the treatments by nano fat

Objectives: To discuss the various treatment modalities for nanofat

Introduction: Ever since the advent of nanofat, its regenerative potential and ease of use have propelled its growing use in a variety of conditions. In this debate, we go over some of these uses and their outcomes

Materials / method: Some of the uses for nanofat include, facial rejuvenation, sun damaged skin, dark circles, scars, vaginal atrophy etc. These indications are treated by intradermal or subcutaneous injections, with or without prp and other modalities.

Results: The results of nanofat are almost universally promising. However more research is needed to standardize treatment regimens.

Conclusion: Nanofat offers exciting opportunities for rejuvenation and regeneration.

Declaraciones

¿Ha recibido algún tipo de financiamiento para realizar su investigación sobre esta temática?
No

¿Ha recibido algún pago, honorario u otra compensación por su trabajo acerca de esta investigación?
No

¿Tiene vínculos financieros con alguna entidad que podría llegar a competir estrechamente con los medicamentos, materiales o instrumentos tratados en su investigación?
No

¿Posee o ha solicitado una patente relacionada a los materiales, productos o instrumentos utilizados en su investigación?
No

Este trabajo no cuenta con el apoyo de ningún financiamiento directo o indirecto. El autor asume plena responsabilidad sobre el mismo.

Round table discussion

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IMCAS Asia class Programa

FOCO EN S32

Enriching the debate on nano fat

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