Objectives: The new compact Q-graft® system provides the possibility to combine the harvest of adipose tissue with the isolation and concentration of SVF cells in one closed sterile environment in a standardized manner.
Introduction: Adipose tissue is a known source of stromal vascular fraction (SVF) cells. They are a promising tool for regenerative medicine and tissue engineering. Today laboratories have established many differing manual SVF-isolation procedures. These methods rely on laminar flow benches to ensure a sterile working environment. In order to be able to consecutively harvest adipose tissue and isolate the SVF in the operating theatre during one operation, a different solution is necessary.
Materials / method: In combination with a body-jet the adipose tissue can be suctioned right into the Q-graft® system. Here it is digested with Humanase™, a blend of proteolytic GMP grade enzymes, under constant mixing and heating to 37 °C. Through a series of steps of washing and filtration the SVF cells can be collected as a suspension in Ringer’s solution. The suspension of the SVF can either be concentrated in the device by cross-flow filtration or by one step of centrifugation using a special sterile Q-graft® centrifugation set.
Results: The Q-graft® system is able to thoroughly separate the SVF cells from the adipocyte fraction of the adipose tissue. Preliminary results show, that with just one step of centrifugation the Q-graft® system was able to isolate an average of 36.3E+04 SVF cells per ml processed tissue. An average of 17.4 % of the cells reached plastic adherence and were CD34-positive.
Conclusion: No bacterial contamination was found in the tested cell suspensions. In ongoing work the CFU content, population doubling time of the isolated adMSC, as well as their mesenchymal differentiation potential are being determined. Thus, the successful isolation of the SVF in the closed Q-graft® system can clear the way for the clinical application of this regenerative cell fraction.
Declaraciones
¿Ha recibido algún tipo de financiamiento para realizar su investigación sobre esta temática?
Si
Por favor especifique las entidades (individuo, empresa, sociedad): This work was financially supported by the European Union (EFRE) and the Federal State of Mecklenburg-Vorpommern (Jet Stem Cell Collector, V-630-S-120-2011/122 & V-630-F-120-2011/123; ARENA, TBI-V-1-003-VBW-001)
¿Ha recibido algún pago, honorario u otra compensación por su trabajo acerca de esta investigación?
Si
Por favor especifique las entidades (individuo, empresa, sociedad): Employed at human med AG for parts of the study.
¿Tiene vínculos financieros con alguna entidad que podría llegar a competir estrechamente con los medicamentos, materiales o instrumentos tratados en su investigación?
No
¿Posee o ha solicitado una patente relacionada a los materiales, productos o instrumentos utilizados en su investigación?
No
Este trabajo es presentado gracias al apoyo de: This work was financially supported by the European Union (EFRE) and the Federal State of Mecklenburg-Vorpommern (Jet Stem Cell Collector, V-630-S-120-2011/122 & V-630-F-120-2011/123; ARENA, TBI-V-003-VBU-001)