Objectives: This research focuses on a biologically active, regenerative alternative for sustained periorbital rejuvenation.
1. Evaluate the Efficacy of ACF Filler
2. Comparison with other autologous-derived materials (such as Coleman fat, nanofat, and ACF).
3. Analyze Patient Satisfaction and Clinical Outcomes
4. Investigate the Regenerative and Biostimulatory Potential of ACF
5. Ensure Safety and Minimize Complications
6. Validate ACF as a Long-Lasting Alternative to Traditional Fillers
7. What to bring from Autologous-Derived Materials to the Future of Regenerative Biomaterials

Introduction: Infraorbital dark circles are a prevalent aesthetic concern worldwide​. This condition arises from anatomical and physiological factors​. The periorbital skin is among the thinnest in the body and, with age-related collagen loss, becomes increasingly translucent—revealing the underlying vascular​. Concurrently, atrophy of periorbital fat pads and surrounding support structures leads to depression and shadowing​. By correcting root structural causes rather than merely masking symptoms, these approaches offer the potential for more resolution of infraorbital dark circles​.

Materials / method: For Animal Study:
Characterization Methods: Histology, Immunostaining, Western blotting, cell viability assays.
Proteomic Analysis: Evaluation of bioactive components in ACF.
Evaluations：Collagen synthesis/degradation, Skin barrier function, Skin filling effectiveness, Collagen remodeling process, dermal thickness, fibroblast expression, antioxidase expression
For Clinical Study: Subjects with dark circles.
Intervention: Single session of Intradermal ACF injection with 8 months follow-up.
Outcome measures: Clinical improvement assessed via Global Aesthetic Improvement Scale (GAIS)

Results: 1. Patient satisfaction:
86% reported being "highly satisfied" or "satisfied." Improvements included brighter, tighter, and smoother infraorbital skin.
2. Clinical outcome (GAIS evaluation):
Over 97% rated as "improved,or above by three independent surgeons. No irregularities or lumps observed during follow-up.
3. ACF-derived adipokines contained anti-inflammatory, skin barrier- and lipidbiosynthesis-related components. ACF contained nonviable cells and high levels of collagen I, collagen IV, and laminin. Fibroblasts and procollagen significantly increased in ACF.

Conclusion: ACF filler is an innovative intradermal approach developed to effectively address the thin region. Derived from adipose tissue, ACF is an extracellular matrix collagen scaffold enriched with adipokines, promoting bio-stimulation and tissue regeneration. It offers a sustained-release mechanism that enhances collagen synthesis and protects skin barrier.
Clinically, ACF represents a promising autologous filler for periorbital rejuvenation, improved skin quality, and minimized risks. ACF as a valuable regenerative solution and bring scientific messages for aesthetics and anti-aging treatments.

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