Objectives: Recent technology allows clinicians to mechanically disaggregate fat tissue into small fat particles known as Nanofat (NF). The present study aims to:
evaluate the cell yield obtained from NF generation in comparison to traditional methods involving enzymatic dissociation (SVF).
Materials / method: NF preparations were characterized by cell content and viability, based on DNA quantification and image cytometry, respectively. DNA analysis was also used to determine the cell content in unprocessed dry Lipoaspirate (LA) and native adipose tissue (Ex-AT). To evaluate cell yield, the authors compared the number of cells recovered from one gram of LA between SVF and NF preparations, and subsequently determined the final cell inoculum obtained following their respective protocols.
Results: The data showed that NF samples presented a cell burden of 7.3 million cells/g, close to 80% of unprocessed dry LA, and 70% of native excised adipose tissue (Ex-AT). Moreover, cell viability was not altered by mechanical disaggregation in NF samples compared to unprocessed dry LA. NF samples exhibited a cell yield of 6.63 million cells/g LA, whereas SVF preparations resulted in only 0.68 million cells/g LA. The final cell inoculum obtained from SVF isolation was 120 million cells and it required 200-250 cc of raw LA as starting material, whereas NF preparation resulted in 125 million cells wit
Conclusion: Mechanical disaggregation offers a better cell inoculum than conventional enzymatic dissociation methods by using ten-times less fat tissue as starting material and delivering a higher cell yield.
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