摘要

Objectives: 1. To understand that exosomes derived from adipose stem cells (ASCE) can be a paradigm shift in regenerative aesthetics & therapeutics 2. To demonstrate that ASCE can reduce severe inflammation and improve the barrier reconstruction in skin and scalp 3. To prove that ASCE can make rejuvenate of scalp and prevent hair loss Introduction: Exosomes are nano-sized (30 - 200 nm in diameter) lipid bilayered vesicles secreted by most cell types. Adipose Stem Cell-derived Exosomes (ASCE) are emerging as a promising cell-free therapy for the treatment of hair loss and scalp rejuvenation. ASCE has been tested for in vitro, in vivo, & clinical studies to prove its efficacy for hair loss. To understand that adipose stem cell exosome (ASCE) can be a paradigm shift in regenerative aesthetics & therapeutics. To know that ASCE can make scalp rejuvenation and hair loss prevention. Materials / method: ASC-Exosomes were isolated from the serum-free conditioned media by sequential filtration and characterized as recommended by the International Society for Extracellular Vesicles (ISEV). The effects of ASC-exosomes were demonstrated from a variety of in vivo, in vitro, & clinical studies against dermal papilla cells and human hair follicles, in order to show longer hairs and better hair bulb shape. In our clinical study, 29 subjects were treated with microneedling procedure with topical ASCE treatment for 6 months. Results: ASCE labeled with green fluorescence was easily up-taken by human dermal papilla cells in vitro. ASCE could reduce by up to 20% the level of cellular aging of human dermal papilla cells from UVB irradiation in vitro. ASCE could effectively increase by 10-20 % the length and thickness of human hairs derived from ex vivo human hair follicles for 8 days, which was superior to minoxidil. ASCE-containing formulation showed strong improvement in clinical cases of androgenic alopecia. The ASCE-containing formulation showed the maintenance of the shape of human hair follicles from an ex-vivo study. Conclusion: ASCE may serve as next-generation technology in terms of regeneration and anti-inflammation for scalp and hair.