利益冲突声明

您有否接受任何资金来支持研究这个主题？
否

您是否接受过关于这项研究的任何酬金或其他报酬？
否

你是否和任何与您的研究所涉及的药物，材料或工具有密切联系的实体存在财务关系？
否

你是否拥有或者您已经为您此研究中的工具，药物或材料申请任何专利？
否

这项工作没有任何直接或间接的资金支持。由作者自己承担责任。

利益冲突声明

您有否接受任何资金来支持研究这个主题？
否

您是否接受过关于这项研究的任何酬金或其他报酬？
否

你是否和任何与您的研究所涉及的药物，材料或工具有密切联系的实体存在财务关系？
否

你是否拥有或者您已经为您此研究中的工具，药物或材料申请任何专利？
否

这项工作没有任何直接或间接的资金支持。由作者自己承担责任。

Objectives: Recent technology allows clinicians to mechanically disaggregate fat tissue into small fat particles known as Nanofat (NF). The present study aims to:
evaluate the cell yield obtained from NF generation in comparison to traditional methods involving enzymatic dissociation (SVF).

Materials / method: NF preparations were characterized by cell content and viability, based on DNA quantification and image cytometry, respectively. DNA analysis was also used to determine the cell content in unprocessed dry Lipoaspirate (LA) and native adipose tissue (Ex-AT). To evaluate cell yield, the authors compared the number of cells recovered from one gram of LA between SVF and NF preparations, and subsequently determined the final cell inoculum obtained following their respective protocols.

Results: The data showed that NF samples presented a cell burden of 7.3 million cells/g, close to 80% of unprocessed dry LA, and 70% of native excised adipose tissue (Ex-AT). Moreover, cell viability was not altered by mechanical disaggregation in NF samples compared to unprocessed dry LA. NF samples exhibited a cell yield of 6.63 million cells/g LA, whereas SVF preparations resulted in only 0.68 million cells/g LA. The final cell inoculum obtained from SVF isolation was 120 million cells and it required 200-250 cc of raw LA as starting material, whereas NF preparation resulted in 125 million cells wit

Conclusion: Mechanical disaggregation offers a better cell inoculum than conventional enzymatic dissociation methods by using ten-times less fat tissue as starting material and delivering a higher cell yield.

利益冲突声明

您有否接受任何资金来支持研究这个主题？
否

您是否接受过关于这项研究的任何酬金或其他报酬？
否

你是否和任何与您的研究所涉及的药物，材料或工具有密切联系的实体存在财务关系？
是
请注明名称（个人，公司，学会等等）: Tulip Medical

你是否拥有或者您已经为您此研究中的工具，药物或材料申请任何专利？
否

这项工作没有任何直接或间接的资金支持。由作者自己承担责任。

利益冲突声明

您有否接受任何资金来支持研究这个主题？
否

您是否接受过关于这项研究的任何酬金或其他报酬？
否

你是否和任何与您的研究所涉及的药物，材料或工具有密切联系的实体存在财务关系？
否

你是否拥有或者您已经为您此研究中的工具，药物或材料申请任何专利？
否

这项工作没有任何直接或间接的资金支持。由作者自己承担责任。