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RULES FOR SUCCESS IN AUTOLOGOUS FAT GRAFTING

Focus program

Room: Webinar
Date: Wednesday 24 July 2024 at 11:00 to 12:30
Format: FOCUS SESSION > lectures covering a major topic of the congress

Chair

Prof Guy MAGALON

Plastic Surgeon

France

Lectures of the session

Hours	Speakers	Lecture title	Abstract	Number
11:00	Prof Guy MAGALON	Introduction		138675
11:05	Dr Regis ROCHE	Fat grafting efficiency: crucial importance of preparation	View	138677
11:25		Discussion and Q&A with the audience		138680
11:33	Dr Sophie MENKES	Keys to a successful facial rejuvenation: effective protocols and smart combinations		138676
11:53		Discussion and Q&A with the audience		139411
12:01	Prof Pietro GENTILE	The use of fat grafting enhanced with PRP and SVF in face and breast soft tissue defects		138678
12:21		Discussion and Q&A with the audience		139410

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Prof Guy MAGALON

Plastic Surgeon

France

Other author(s) :

Introduction

Dr Regis ROCHE

PhD - Research

France

Other author(s) :

Fat grafting efficiency: crucial importance of preparation

Objectives: Understand the rules for preparing adipose tissue for an effective autologous fat grafting

Introduction: Autologous fat grafting is widely used throughout the world in plastic and reconstructive surgery, thanks to its very natural results and low complications. However, the major problem with the technique remains tissue resorption after injection, leading to a significant reduction in the volume injected.
Indeed, the conditions under which the adipose tissue is harvested, prepared and reinjected will considerably influence its survival during and after surgery, which can vary from 30% to 80% depending on the techniques and volumes used.

Results: Despite the relatively small number of specific studies on adipose tissue preparation techniques and their relationship to survival, several major concepts have emerged over the last 10 years, leading to a consensus on the best way to harvest and prepare adipose tissue for autologous grafting: infiltration, aspiration, injection and other preparation techniques are compared in this presentation, to highlight the essential rules required for effective adipose tissue grafting.

Conclusion: In a comparative study with several conventional techniques, we show that compliance with these rules results in grafting rates of over 90%.

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work is presented thanks to the support of: Neosyad company

Discussion and Q&A with the audience

Dr Sophie MENKES

Switzerland

Other author(s) :

Keys to a successful facial rejuvenation: effective protocols and smart combinations

Discussion and Q&A with the audience

Prof Pietro GENTILE

Plastic Surgeon

Italy

Other author(s) :

The use of fat grafting enhanced with PRP and SVF in face and breast soft tissue defects

Objectives: This study aimed to report the in vivo and in vitro results, represented by fat volume maintenance and AD-SVFs amount, obtained by four different procedures of AD-SVFs isolation and A-FG purification based on centrifugation, filtration, centrifugation with filtration, and enzymatic digestion.

Introduction: Currently, several techniques for autologous fat graft (A-FG) preparation aimed at obtaining purified tissue exist. Both mechanical digestions via centrifugation, filtration, and enzymatic digestion were considered the most effective with different impacts in terms of adult adipose-derived stromal vascular fraction cells (AD-SVFs) amount that volume maintenance.

Materials / method: A prospective, case-control study was conducted. In total, 80 patients affected by face and breast soft tissue defects were treated with A-FG and divided into four groups: n=20 were treated with A-FG enhanced with AD-SVFs obtained by enzymatic digestion (study group 1 [SG-1]); n=20 were treated with A-FG enhanced with AD-SVFs obtained by centrifugation with filtration (SG-2); n=20 were treated with A-FG enhanced with AD-SVFs obtained by only filtration (SG-3); n=20 were treated with A-FG obtained by only centrifugation according to the Coleman technique (control group [CG]).

Results: Starting with the same amount of fat analyzed (20 mL), 50,000 ± 6956 AD-SVFs/mL were obtained in SG-1; 30,250 ± 5100 AD-SVFs/mL in SG-2; 33.333 ± 5650 AD-SVFs/mL in SG-3, while 500 AD-SVFs/mL were obtained in CG. In patients treated with A-FG enhanced with AD-SVFs obtained by automatic enzymatic digestion, a 63% ± 6.2% maintenance of fat volume restoring after 1 year was observed compared with 52% ± 4.6% using centrifugation with filtration, 39% ± 4.4% using only centrifugation (Coleman), and 60% ± 5.0% using only filtration.

Conclusion: In vitro AD-SVFs cell analysis indicated that filtration was the most efficient system-between mechanical digestion procedures-thanks to the highest amount of cells obtained with fewer cell structure damage, producing in vivo, the most volume maintenance after 1 year. Enzymatic digestion produced the best number of AD-SVFs and the best fat volume maintenance.

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability

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IMCAS Academy International Program

RULES FOR SUCCESS IN AUTOLOGOUS FAT GRAFTING

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Lectures of the session

IMCAS Academy International
Program