摘要

Objectives: To evaluate the wound-healing efficacy of the AcuCool™ system combined with epidermal growth factor (EGF) in a full-thickness rat skin wound model. Introduction: Impaired wound healing remains a major clinical challenge, particularly when inflammation is prolonged and tissue regeneration is delayed. This study evaluated the therapeutic efficacy of AcuCool™, a novel platform combining controlled CO₂ cryotherapy with intradermal epidermal growth factor (EGF) delivery, for enhancing wound repair. Materials / method: A full-thickness excisional wound model was established in Sprague Dawley rats. Animals were divided into three groups: untreated control, microneedling-assisted EGF delivery (MTS+EGF), and AcuCool™ device-assisted EGF delivery (Device+EGF). Wound closure was assessed macroscopically on Days 0, 3, 7, and 14. On Day 14, tissue samples were harvested for histological analysis using hematoxylin and eosin and Masson’s trichrome staining. Inflammatory and regenerative responses were evaluated by ELISA, nitric oxide assay, immunofluorescence staining, and qRT-PCR analysis. Results: The Device+EGF group showed significantly accelerated wound closure compared with the control and MTS+EGF groups. Histological analysis demonstrated narrower wound gaps, improved re-epithelialization, reduced inflammatory cell infiltration, and increased collagen deposition. Device+EGF treatment significantly decreased inflammatory and oxidative markers, including TNF-α, IL-1β, MCP-1, and nitric oxide. In addition, regenerative and remodeling-related markers, including TGF-β1, Collagen I, and Vimentin, were significantly upregulated, indicating enhanced extracellular matrix formation and tissu Conclusion: AcuCool™-assisted CO₂ cryotherapy combined with EGF delivery significantly promotes wound healing through coordinated anti-inflammatory, antioxidant, and regenerative mechanisms. This dual-action platform may represent a promising non-invasive strategy for improving wound repair, although further studies in chronic wound models are required to confirm its clinical applicability.