Objectives: Lecture Objectives on Secretomes
*Define secretomes and their components (growth factors, cytokines, extracellular vesicles, etc.).
*Highlight the biological processes mediated by secretomes, such as cellular communication, angiogenesis, and tissue repair.
*Discuss the differences between secretomes and regenerative therapies like exosomes or stem cell therapy.
*Explore the mechanisms of action of secretomes in tissue repair, anti-inflammation, and collagen production.
*Present their role in cellular signalling, focusing on their ability to activate dormant cells and regenerate tissue

Introduction: Definition: Secretomes are the substances secreted by cells into their surrounding environment. In regenerative medicine, the focus is on secretomes derived from mesenchymal stem cells (MSCs) or adipose-derived stem cells (ADSCs).
Components:
Growth Factors: Stimulate cell growth, proliferation, and differentiation (e.g., VEGF, TGF-β).
Cytokines: Modulate immune responses and reduce inflammation.
Extracellular Vesicles: Carry genetic material (microRNA, mRNA) and proteins to influence other cells.
Proteins and Enzymes: Aid in tissue remodeling and repair.

Materials / method: Provide an overview of how secretomes are applied in various aesthetic procedures:
Skin rejuvenation: Enhancing skin texture, elasticity, and hydration.
Hair restoration: Stimulating follicle regeneration and reducing shedding.
Scar management: Improving wound healing and reducing pigmentation.
Post-procedure recovery: Supporting tissue healing after treatments like microneedling, laser therapy, or PRP.
Showcase clinical case studies to demonstrate results and highlight best practices.

Results: Skin Aging: Studies show that secretomes from MSCs improve skin hydration, elasticity, and reduce signs of aging.
Hair Regrowth: Early research indicates that secretomes boost follicle regeneration, especially in androgenetic alopecia.
Combination Approaches: Clinical trials are exploring secretome-enhanced PRP and exosome therapies for better outcomes.
Advantages of Secretomes
Non-Invasive: Delivered via microneedling, injectables, or topicals, secretomes are minimally invasive.
Safety: Secretomes do not contain live cells, reducing the risk of rejection or immune reactions.
Potent Results:

Conclusion: Educational Takeaways

Empower practitioners with actionable knowledge to integrate secretomes into their clinical practice.
Provide a clear framework for patient selection, treatment protocols, and expected outcomes.
Inspire attendees to stay updated on scientific advancements and adopt evidence-based practices.

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Objectives: Understanding pigmentary issues of face associated with contour deformities
Appreciate role of stem cells in pigmentation regulation of melanocytes

Introduction: Facial contour deformities associated with pigmentary changes are of major concern for plastic surgeons both being important and difficult in treating such issues. No definite ideal treatment option is available to address simultaneously both the contour defect as well as related pigmentation. The aim of the current study is to compare long term effects of conventional adipose tissue grafting and ex-vivo expanded MSCs enriched adipose tissue grafting for the treatment of contour deformities with pigmentary changes on face.

Materials / method: Eighty patients of contour deformities of face with hyperpigmentation were recruited after informed consent. Two techniques i.e. conventional fat grafting (C-FG) and fat grafts enriched with expanded adipose stem cells (FG-ASCs) were used to address the pigmentation. Both techniques were explained to patients and enrolled patients were divided into two groups i.e. C-FG and FG-ASCs as per patients’ choice and satisfaction. FG-ASCs group treated with fat grafts enriched with expanded ASCs while C-FGs group treated with conventional fat grafting Patients followed for 12 months for improvement.

Results: Mean age of patients was 24.42(±4.49), 66 patients were females. Forehead was involved in 61.20% cases, cheek in 21.20% cases, chin in 11.20% cases and nose in 6.20% cases. In GF-ASCs group, the integrated color density (ICD) was decreased (1.08×106 ±4.64×105) as compared to C-FG group (2.80×105±1.69×105). Patients treated with fat grafts enriched with expanded ASCs were significantly more satisfied as compared to patients treated with conventional fat grafting only.

Conclusion: ASCs enriched autologous fat grafting is preferred option for improving the contour deformities related increased pigmentation of face skin.

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Objectives: Exosomes and related technologies are booming research fields in regenerative medicine. Recent discoveries unveiled their potential in improving cellular health-span and skin aesthetic quality. This lecture will help the audience gain insights on:
1). Updated science-based evidence on Exosomes, Extracelullar vesicles derived from non-mammalian species, Bio-engineered vesicles, and their potential application in regenerative aesthetics and health-span improvement.
2). New imaging techniques to characterize and visualize exosomes.
3). Biomarkers and longevity hallmarks being investigated.

Introduction: Exosomes, small vesicles carrying bioactive molecules such as RNAs and signaling peptides, are showing promise in regenerative medicine and aesthetics. Early research suggests they may rejuvenate tissues, accelerate wound healing, and reduce inflammation. In addition, recent discoveries demonstrated their potential to impact human longevity hallmarks. Exosome-related technologies such as extracellular vesicles from various species, bioengineered vesicles, and liposomal encapsulated biomaterials are emerging, bringing new avenues for therapeutic and aesthetic applications.

Materials / method: Reviews of updated clinical trials and scientific literatures will be shared for knowledge updates. Human dermal fibroblasts were cultured, conditioned media was harvested and concentrated, Nanotracker was adopted to visualize and quantify exosomes and vesicles from different species. Transmission electron microscope was used to visualize more detailed vesicle structures. The membrane of exosomes was tagged with a fluorescent dye in order to visualize the secretion and uptake process between live cells. qPCR and multiplex were used to investigate biomarkers for regeneration and longevity.

Results: Both human mesenchymal stem cell-derived exosomes and extracellular vesicles from non-mammalian source could impact biosignatures of cellular activities, including cell signaling, proliferation, migration, and beyond. Differentiated biomarkers for regeneration and cellular health were impacted by vesicles derived from various species, or through different manufacturing processes. While these findings are promising, there is a lack of standardized process of exosome production, characterization, and double-blind placebo-controlled clinical trials, future research and investigation is required.

Conclusion: Exosomes and adjacent technologies have demonstrated promising results in regenerative aesthetics and tissue health-span. Despite these promising avenues, comprehensive scientific and clinical validation is needed to fully understand the efficacy and safety of exosome-related treatments in promoting regeneration, longevity, and health benefits.​

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