Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability

Objectives: Recent technology allows clinicians to mechanically disaggregate fat tissue into small fat particles known as Nanofat (NF). The present study aims to:
evaluate the cell yield obtained from NF generation in comparison to traditional methods involving enzymatic dissociation (SVF).

Materials / method: NF preparations were characterized by cell content and viability, based on DNA quantification and image cytometry, respectively. DNA analysis was also used to determine the cell content in unprocessed dry Lipoaspirate (LA) and native adipose tissue (Ex-AT). To evaluate cell yield, the authors compared the number of cells recovered from one gram of LA between SVF and NF preparations, and subsequently determined the final cell inoculum obtained following their respective protocols.

Results: The data showed that NF samples presented a cell burden of 7.3 million cells/g, close to 80% of unprocessed dry LA, and 70% of native excised adipose tissue (Ex-AT). Moreover, cell viability was not altered by mechanical disaggregation in NF samples compared to unprocessed dry LA. NF samples exhibited a cell yield of 6.63 million cells/g LA, whereas SVF preparations resulted in only 0.68 million cells/g LA. The final cell inoculum obtained from SVF isolation was 120 million cells and it required 200-250 cc of raw LA as starting material, whereas NF preparation resulted in 125 million cells wit

Conclusion: Mechanical disaggregation offers a better cell inoculum than conventional enzymatic dissociation methods by using ten-times less fat tissue as starting material and delivering a higher cell yield.

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
Yes
Please specify entities (individual, company, society): Tulip Medical

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability

Disclosures

Did you receive any funding to support your research for this TOPIC?
No

Were you provided with any honoraria, payment or other compensation for your work on this study?
No

Do you have any financial relationship with any entity which may closely compete with the medications, materials or instruments covered by your study?
No

Do you own or have you applied for any patents in conjunction with the instruments, medications or materials discussed in your study?
No

This work was not supported by any direct or non direct funding. It is under the author's own responsability